The capital must be invested or actively in the process of being invested when the petition is filed. An unsigned or unclosed sale means there are no funds to invest yet, so filing would be premature.
Money must exist before it can be at risk
Hypothetical example: a Quispamsis dentist has accepted an offer for her practice with closing set for three months out, and the regional center is pressing her to sign and file now to secure a place in the project. The petition must show that the required capital has been invested or is in the process of being invested, which means actually committed, not promised from a sale that has not occurred. A signed subscription agreement with no funds behind it does not meet the test.
She can complete the source-of-funds documentation now, negotiate the subscription documents and line up the escrow so that the investment can be made promptly at closing. The petition should follow the transfer. If the regional center's project has a genuine capacity limit, that is a commercial matter to negotiate; it does not change the legal requirement that the capital exist and be committed when she files.